NANSLO Remote Lab Activity: Enzyme

This NANSLO lab activity is self-contained and can be used in second semester general biology with lab. In this activity, glucose oxidase is injected into a sugar solution resulting in a color change which is detected/quantified by a spectrometer. Reactions can be performed six different times to see how conditions affect rates. The lab activity includes background information on enzymes, how they are affected by certain variables, how colorimetric enzyme reaction are used to explain enzyme activity, how to determine the effect of temperature on enzymatic activity, how to determine the effect of substrate concentration on enzymatic activity, and describes how to graph quantitative data collected. The lab activity includes pre-lab exercises, lab exercises, and summary questions.
Date:
2014-08
Primary Material Type:
Assignment
Institution:
Western Interstate Commission for Higher Education
Funding Source:
TAACCCT Round 2
Subjects:
NANSLO Remote Lab Activity: Enzyme, describes enzymes and the affect of certain variables on them, look at the effects of temperature change on enzyme activity and describes how to collect and graph quantitative data, laboratory exercises

Industry / Occupation

Industry Partner:
Health Care
Industry Sector:
Health Care and Social Assistance (62)
Occupation:
Healthcare Practitioners and Technical Occupations (29-0000)

Education / Instructional Information

Instructional Program:
Health Professions and Related Clinical Sciences (51)
Credit Type:
  • Credit
Credential Type:
  • Associate Degree
  • Bachelors Degree
Educational Level of Materials:
  • 1st year Community College or equivalent
Time Required:
2 Hrs connected to laboratory through the Internet plus pre-lab and post-lab work
Language:
English (United States)
Interactivity Type:
Active - requires user to respond to prompts, questions, etc.
Quality Note:
Curriculum expert in this discipline worked with Discipline Panel of faculty to define learning objectives needed for this lab activity. During development, teleconferences were held to discuss lab activity being developed. Draft activity was posted to listserv for review by Discipline Panel and other faculty in that discipline. Based on feedback, lab activity was modified prior to posting as final. Experts in remote web-based science labs who are also science faculty worked together to define the web-enabled interface design used by students to perform the lab activity via the Internet and created the lab setup (robotics, scientific equipment, and items needed to complete the lab activity) testing it with lab technicians and others prior to launching the final lab activity.
Quality of Subject Matter was assured by:
  • Consultations during development of instructional materials
Quality of Online/Hybrid Course Design assured by:
  • None
Course Note:
Second semester General Bioloty with lab course

Copyright / Licensing

Primary License:
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.