ABO Forward and Reverse - Tube Method

In the gel test, ABO forward grouping and D antigen typing are performed in the Anti-A, Anti-B, and Anti-D microtubes, which contain the specific antibody incorporated into the gel. ABO reverse grouping is performed in the Buffered Gel microtubes. Agglutinated red blood cells become trapped in the gel at various levels within the microtube, depending upon the size of the agglutinates. Free nonagglutinated red cells pass through the gel and form a button of red blood cells on the bottom of the microtube. The control microtube must be negative for the results to be valid.
Primary Material Type:
Lake Area Technical Institute

Industry / Occupation

Industry Partner:
Prairie Lakes Healthcare System
Industry Sector:
Health Care and Social Assistance -- Ambulatory Health Care Services -- Medical and Diagnostic Laboratories (6215)
Healthcare Practitioners and Technical Occupations -- Medical and Clinical Laboratory Technicians (29-2012)

Education / Instructional Information

Instructional Program:
Health Professions and Related Clinical Sciences (51)
Credit Type:
  • Credit
Credential Type:
  • Associate Degree
Educational Level of Materials:
  • 2nd Year Community College or equivalent
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Active - requires user to respond to prompts, questions, etc.
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  • Participation as an ongoing member of team developing the instructional materials
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  • Custom Quality Rubric
Course Note:
Medical Laboratory Technician

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