Title: E4 Quiz
Course: Lab Instruments II > Section: E4: Meetings 9,10,11

Quiz over the objectives for Experiment Set 4.

Q: An HPLC injection loop is designed for more sample to be injected than the loop is rated to hold.
- True
- False
A: True

Q: HPLC systems can generate backpressures of up to 5000 psi under normal working conditions.
- True
- False
A: True

Q: Helium sparging is used to replace gases in HPLC solvents because helium will stay dissolved in the solvents.
- True
- False
A: False

Q: Capillary electrophoresis utilizes a gel slab charged between plastic plates.
- True
- False
A: False

Q: In gel electrophoresis, a 2+ cation will migrate faster than a 1+ cation having the same diameter.
- True
- False
A: True

Q: In CZE, a larger cation will migrate faster than a smaller cation having the same charge.
- True
- False
A: False

Q: Which type of separation system should be utilized for a mixture of neutral, polar molecules?
- Gel electrophoresis
- Ion chromatography
- Normal phase chromatography
- Reverse phase chromatography
A: Normal phase chromatography

Q: Which of the following lists the HPLC components in the correct order of solvent flow?
- gradient programmer, pump, injection loop, guard column, analytical column
- pump, gradient programmer, guard column, injection loop, analytical column
- gradient programmer, injection loop, guard column, analytical column, pump
- pump, injection loop, gradient programmer, analytical column, guard column
A: gradient programmer, pump, injection loop, guard column, analytical column

Q: Which of the following lists the steps associated with running solvent through an HPLC column in the proper order?
- flush, sample separation, equilibration
- equilibration, sample separation, flush
- sample separation, equilibration, flush
- equilibration, flush, sample separation
A: equilibration, sample separation, flush

Q: Which of the following sample preparation steps should be taken before injecting any HPLC sample?
- Degassing
- Distilling
- Extracting
- Filtering
A: Filtering

Q: Which one of the following is NOT a reason to use gradient elution?
- Increase resolution of analyte peaks
- Decrease retention time of late eluting peaks
- Increase retention time of fast eluting peaks
- All of the above are potential reasons to use gradients.
A: All of the above are potential reasons to use gradients.

Q: Which type of detector used to create an HPLC chromatogram is relatively universal but not very sensitive?
- Conductivity detector
- Fluorometer
- Refractometer
- UV spectrometer
A: Refractometer

Q: Which of the following best describes the chromatogram produced by UV-diode array detector?
- emission of light versus time
- absorbance of light versus time
- emission of light versus time and versus wavelength
- absorbance of light versus time and versus wavelength
A: absorbance of light versus time and versus wavelength

Q: Which of the following could be the cause of low pressure in an HPLC system?
- Dirty filters
- Old guard columns
- Air bubbles in the system
- Incompatible mobile and stationary phases
A: Air bubbles in the system

Q: What is the term for the phenomenon in which cations moving through the column towards the cathode creates a flow that moves all the components of the mobile phase in the same direction?
- Electrostatic flow
- Electroosmotic flow
- Osmotic pressure flow
- Osmoelectrophretic flow
A: Electroosmotic flow