Title: Discussion Forum Description: E4 Gradient Elution and 3D Chromatograms
Course: Lab Instruments II > Section: E4: Meetings 9,10,11

1. Choose one of the topics listed below. Once half of the class has posted on one topic, then the remaining students should post on the other topic. Each student must post a unique reference.
  1. Gradient Elution: Find a series of two or more HPLC chromatograms for the same sample in which at least one of the chromatograms is a result of gradient elution. Summarize the elution programs used and how they affected the retention times and resolution. Include the reference link.
  2. 3D Chromatograms: Find an HPLC chromatogram created using a diode array detector. Describe how it looks, including the axes labels, type of components separated, and if normal or reverse phase. Include the reference link.
2. Provide a substantive, thoughtful response to a post on the topic that you did not choose to summarize for step 1 (i.e. if you found a gradient elution, then comment on a 3D chromatogram post and vice versa).