Title: E3 Quiz
Course: Lab Instruments II > Section: E3: Meetings 6,7

Quiz over the objectives for Experiment Set 3.

Q: In anion exchange chromatography, which is true?
- The stationary phase is negatively charged and will bind with anions of the sample for separation.
- The stationary phase is negatively charged and will bind with cations of the sample for separation.
- The stationary phase is positively charged and will bind with anions of the sample for separation.
- The stationary phase is positively charged and will bind with cations of the sample for separation.
A: The stationary phase is positively charged and will bind with anions of the sample for separation.

Q: Which type of chromatography uses a nonpolar organic mobile phase instead of an aqueous one?
- Anion exchange chromatography
- Cation exchange chromatography
- Gel permeation chromatography
- Gel filtration chromatography
A: Gel permeation chromatography

Q: Which type of stationary phase loses its exchange capacity due to full protonation at low pH’s?
- SCX
- WCX
- SAX
- WAX
A: WCX

Q: What is the term for the type of elution method that uses the same mobile phase composition throughout the sample separation procedure?
- Gradient elution
- Gradual elution
- Isocratic elution
- Isotonic elution
A: Isocratic elution

Q: The calcium ion, Ca^2+, will likely have a longer retention time than the potassium ion, K^+ in cation exchange chromatography.
- True
- False
A: True

Q: As ionic strength of the mobile phase increases, the ability of sample components to be retained by the stationary phase increases.
- True
- False
A: False

Q: In size exclusion chromatography, larger components have shorter retention times than smaller components.
- True
- False
A: True

Q: An internal standard should have the same retention time as the analyte.
- True
- False
A: False

Q: In LC, ______ is the term for the mobile phase that enters the column and ______ is the term for the mobile phase mixed with sample components that exits the column.
- eluant; elute
- elute; eluant
- eluate; eluent
- eluent; eluate
A: eluent; eluate

Q: What is the purpose of the suppressor column in an IC system?
- Reduce the volume of sample being separated
- Separate the components of the sample mixture
- Remove contaminates before they enter the main column
- Remove mobile phase ions before they enter the detector
A: Remove mobile phase ions before they enter the detector

Q: Which one of the following does NOT adjust for dead volume?
- Capacity factor
- Adjusted retention time
- Retention time
- Relative retention time
A: Retention time

Q: Which one of the following is NOT affected by small differences in column sizes between runs?
- Adjusted retention time
- Retention time
- Relative retention time
- Void time
A: Relative retention time

Q: Which one of the following statements is true for instrumental quantitative analysis?
- In a single chromatographic run, the component of the mixture in highest concentration will have the greatest peak area.
- In a single chromatographic run, the component of the mixture in highest concentration will have the greatest peak intensity.
- In a single chromatographic run, the component of the mixture in highest concentration will have the greatest peak width.
- In a single chromatographic run, the component of the mixture in highest concentration cannot be determined from the chromatogram alone.
A: In a single chromatographic run, the component of the mixture in highest concentration cannot be determined from the chromatogram alone.

Q: Which of the following is associated with good resolution?
- Large retention times
- Large sample volumes
- Large number of theoretical plates
- Large height equivalent to a theoretical plate
A: Large number of theoretical plates

Q: Which one of the following quantitative methods is utilized when needing to account for variations in sample injection volume from one run to another?
- Response factor
- Internal standard
- Standard additions
- Any of the methods
A: Internal standard