Title: E2 Quiz
Course: Lab Instruments II > Section: E2: Meeting 5

Quiz over the objectives for Experiment Set 2.

Q: In chromatography, the phase that moves is termed the _______ phase.
- liquid
- mobile
- solvent
- travel
A: mobile

Q: In chromatography, the nonmoving phase is termed the _______ phase.
- solid
- solute
- static
- stationary
A: stationary

Q: For LSC, what do the “L” and “S” indicate?
- The mobile phase can be either a liquid or solid.
- The stationary phase can be either a liquid or solid.
- The mobile phase is a liquid and the stationary phase is a solid.
- The stationary phase is a liquid and the mobile phase is a solid.
A: The mobile phase is a liquid and the stationary phase is a solid.

Q: Which one of the following types of chromatography uses differential distribution between a liquid stationary phase and either a liquid or gas mobile phase?
- Adsorption
- Ion-exchange
- Partition
- Size exclusion
A: Partition

Q: Which one of the following types of chromatography uses differential distribution between a stationary phase consisting of polymer beads with small pores and a liquid mobile phase?
- Adsorption
- Ion-exchange
- Partition
- Size exclusion
A: Size exclusion

Q: When two atoms share electrons, the _____ electronegative atom acts as the “electron hog” so that is takes on a partial ______ charge, leaving the _____ electronegative atom with a partial ____ charge.
- more; positive; less; negative
- more; negative; less; positive
- less; positive; more; negative
- less; negative; more; positive
A: more; negative; less; positive

Q: Identify the one FALSE statement below.
- A nonpolar molecule can have polar bonds.
- A nonpolar molecule has an unsymmetrical distribution of charge.
- A polar molecule has a partial positive side and a partial negative side.
- A polar molecule has polar bonds that are not arranged in symmetrical geometric shape.
A: A nonpolar molecule has an unsymmetrical distribution of charge.

Q: Which of the following does NOT apply to normal phase chromatography?
- Silica gel thin layer chromatography is an example configuration.
- Could utilize a mobile phase mixture of hexane and ethyl acetate.
- Consists of a polar stationary phase with a relatively nonpolar mobile phase.
- More polar components of a mixture will travel more than less polar components.
A: More polar components of a mixture will travel more than less polar components.

Q: In paper chromatography, what is the stationary phase?
- cellulose
- filter paper
- petri dish
- water
A: water

Q: In TLC, what is the stationary phase?
- a powdered mixture that has been hardened
- a flat piece of aluminum or glass
- a mixture of liquids
- a beaker
A: a powdered mixture that has been hardened

Q: Identify the FALSE statement regarding instrumental chromatography.
- Produces a chromatogram
- Includes TLC, GC, and HPLC
- Utilizes a column to hold the stationary phase
- Forces the mobile phase through using pressure
A: Includes TLC, GC, and HPLC

Q: Octadecyl silane is a common stationary phase for reverse phase chromatography.
- True
- False
A: True

Q: The further a component travels on a TLC plate, the larger its Rf value will be.
- True
- False
A: True

Q: The value to put in the denominator for the calculation of the Rf value is the distance between the solvent front and the edge of the plate.
- True
- False
A: False

Q: Fractions are the separate volumes of eluate collected from an open column chromatographic separation.
- True
- False
A: True