Title: Midterm Exam
Course: Lab Applications of Microbiology > Section: Course Content

1. Description: Midterm Exam on Experiment 1-7 Objectives
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1. Description: Midterm Exam on Experiment 1-7 Objectives
2. Force Completion: This test can be saved and resumed later
3. Multiple Attempts: This test allows one attempts.

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Q: You learned about the types of containment for working safely with microorganisms. Which of the following best describes the kind of containment that is called primary containment?
- The principle of keeping lab workers in the lab all the time and never letting them go outside.
- The protection of the outside environment from infectious microbes.
- The protection of the people in the lab and the lab environment from infectious microbes
- None of the above
A: The protection of the people in the lab and the lab environment from infectious microbes

Q: Which of the following best describes secondary containment of microorganisms?
- The principle that even if workers stop working in the lab, you can still lock the building to try to keep them working
- It is the protection of the lab workers and the lab environment from infectious microbes.
- It is the protection of the outside environment from exposure to infectious microorganisms, and depends on the design of the laboratory and the availability of equipment.
- None of the above.
A: It is the protection of the outside environment from exposure to infectious microorganisms, and depends on the design of the laboratory and the availability of equipment.

Q: Which of the following is a reason you should put your cell phone away before working in the microbiology lab?
- Because holding it near your face increases your risk of infection
- Because touching your cell phone while working increases the risk of carrying the microorganisms in the lab home with you.
- Because using your cell phone while working could distract you, which could be hazardous.
- All of the above
A: All of the above

Q: Which of the following should be not worn in the microbiology lab?
- Long hair that is not tied back.
- A lab coat
- Safety glasses
- Closed-toed shoes
A: Long hair that is not tied back.

Q: Which of the following is true about biosafety level BSL2?
- It is used to describe the procedures that should be used to handle samples containing pathogens that can be transmitted through fluids, and pose moderate health risks.
- It is the highest safety level you are likely to encounter in your microbiology lab course.
- It is recommended that you wear gloves and safety glasses at this level.
- All of the above.
A: All of the above.

Q: Which of the following is true about the diaphragm of the microscope?
- It lets you adjust the amount of light on the sample
- It holds the lenses of the microscope
- It holds the sample
- You turn it to adjust the focus
A: It lets you adjust the amount of light on the sample

Q: What is the stage of the microscope?
- The place you put the sample
- What you turn to adjust the focus
- The part you adjust to get more light on the sample
- The part that holds the lenses of the microscope
A: The place you put the sample

Q: What is the difference between resolution and magnification?
- Resolution describes how much bigger the image is compared to the sample; magnification is the clarity of the image produced, or how well you can make out the fine details.
- Magnification describes how much bigger the image is compared to the sample; resolution is  the clarity of the image produced, or how well you can make out the fine details.
- Magnifiction describes how much bigger the sample is compared to the image; resolution is the clarity of the image produced, or how well you can make out the fine details.
- None of the above
A: Magnifiction describes how much bigger the sample is compared to the image; resolution is the clarity of the image produced, or how well you can make out the fine details.

Q: It is important to keep the lenses of your microscope clean.
- True
- False
A: True

Q: Which of the following is an important part of media?
- A source of carbon (sugar)
- A source of amino acids
- water
- All of the above
A: All of the above

Q: Microorganisms are rarely found in the environment, so aseptic culture techniques to keep everything sterile are something we do for fun that's really not completely necessary to microbiology.
- True
- False
A: False

Q: The streak plate is a very fast method of inoculating a plate, and is commonly used to get a single colony from a freezer stock of a microorganism.
- True
- False
A: True

Q: Which of the following is not something that you can learn from a viable plate count?
- How much bacteria is in the sample
- How much of the bacteria in the sample is coliform
- Both 1 and 2 are true
- None of the above
A: How much bacteria is in the sample

Q: A normal, countable plate should have how many colonies?
- 5-25
- 25-250
- 250-500
- 500-750
A: 25-250

Q: A plate with over 1000 colonies would be recorded as:
- TFTC
- TMTC
- 1000+
- None of the above
A: TMTC

Q: UV light kills microorganisms by:
- Electric shock
- Making their temperature too high
- Giving them cancer
- Damaging their DNA
A: Damaging their DNA

Q: Besides UV light, microorganisms could be killed by:
- Heat
- Extreme pressure
- Chemical disinfectants
- All of the above
A: Heat

Q: Different microorganisms have different levels of resistance to UV light.
- True
- False
A: True

Q: A mesophile is a:
- Heat-loving microorganism
- Cold-loving organism
- Medium-temperature-loving organism
- None of the above
A: Heat-loving microorganism

Q: Which of the following is a way that temperature can affect bacteria metabolism?
- Enzymes can denature at high temperature
- Lipid membranes can be destroyed at high temperature
- Vital chemical reactions can be slowed down at low temperature
- All of the above
A: All of the above