Title: Ch. 7.3.4 quiz
Course: Lab Safety > Section: Day 5

Adapted from Section on Biological Safety Cabinets in Laboratory Safety by Hill and Finster, 2010 by Wiley

Q: What is the most important laboratory method by which exposure to microbiological agents is prevented?
- Wearing masks
- Wearing gloves
- Containment
- Incineration of waste
A: Containment

Q: Fomites are
- Inanimate objects or surfaces
- Small microbiological organisms
- Agents used to decontaminate surfaces
- Filters using in BSCs
A: Inanimate objects or surfaces

Q: HEPA stands for
- High Energy Particle Accelerator
- High Efficiency Particulate Air
- High Exhaust Product Access
- High Efficiency Plastic Article
A: High Efficiency Particulate Air

Q: HEPA filters are... (Select all that apply.)
- Used to clean the air entering a BSC
- Used to clean the exhaust from a BSC
- Commonly found in face masks
A: Used to clean the air entering a BSC; Used to clean the exhaust from a BSC

Q: BSCs are designed to protect personnel and
- The lab environment
- The contents of the BSC
- The lab environment and the contents of the BSC
- The lab environment but not always the contents of the BSC
A: The lab environment but not always the contents of the BSC

Q: BSCs can typically
- Handle most reagents that are used in chemical hoods
- Handle only very small amounts of volatile chemicals
- Be used as a substitute for a chemical hood
- Handle flammable reagents as long as the LEL is exceeded
A: Handle only very small amounts of volatile chemicals

Q: Face velocities for BSCs should be
- Well below 75 ft/min to avoid turbulence in the cabinet
- Well below 75 ft/min to avoid back-pressure from the HEPA filters
- 75 or 100 ft/min
- Always above 100 ft/min so that no air can escape back into the lab
A: 75 or 100 ft/min

Q: A canopy unit in a BSC
- Helps provide laboratory exhaust when the BSC is off
- Is placed over, but not directly connected to, the BSC
- Removes chemical vapors from the BSC
- All of the above
A: All of the above

Q: Which class of BSC is a glovebox?
- I
- II
- III
- I or II
- II and III
A: III

Q: Laminar flow describes the
- Smooth flow of air in layers down and across the front of the BSC
- Splitting of the exhaust air between recirculated and nonrecirculated paths
- Smooth flow of air across the laminated surface of the BSC
- Flow of air through the HEPA filter
A: Smooth flow of air in layers down and across the front of the BSC

Q: Good practice inside BSCs involves
- Keeping all materials and equipment at least 4 inches from the front, back, and sides of the unit
- Keeping your arms on the grille when working
- Generating aerosols, if necessary to do so, near the back of the unit
- Never using a vacuum system inside a BSC
A: Generating aerosols, if necessary to do so, near the back of the unit

Q: Good practice when using BSCs involves
- Decontamination at least once a week
- Using plastic-backed absorbent paper that drapes across the grille
- Collecting and discarding, or decontaminating, waste carefully
- All of the above
A: Collecting and discarding, or decontaminating, waste carefully