Title: Assignment: LAB - Flowers and Trees (phylogeny)
Course: BIO-182 RTZ

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Select FLOWERS and TREES from the options provided. This week, you will have to print the workbook for this lab; it contains the instructions you need to follow so that the lab makes sense.  There are 24 pages to the workbook. I recommend double-sided printing... and maybe even consider printing two pages to a sheet. The font will be small but it might save toner and paper to do so.

Read the following section: Introduction (pages 1 and 2). This section describes a bit about the biology of Columbines and about the case study (research by Scott Hodges and Michelle Fulton) used in developing this lab.  The hypothesis they tested was that the evolution of nectar spurs led to a rapid diversification of columbine species. The objective of this lab is to further develop your ability to read and use phylogenetic (evolutionary) trees.

DO the following activities:

- Exercise 1: Columbine Flower Traits (aka Designer Flowers). In this first exercise, you are to become familiar with the traits (and mutations) you'll examine in later exercises. There are no questions to answer in this exercise.
- Exercise 2: Watching the Evolutionary Tree Grow (aka Growing Tree). In this activity, you observe the speciation events and relate that to phylogenetic tree. Answer the following questions: 3.1, 4.1, 4.2, & 6.1. Clearly identify the question being answered and use complete sentences in your answers.
- Exercise 3: Building and Reading Trees (aka Simple Evolving Flowers). In this exercise, you cause mutations to happen. Proceed through the activity - all 7 pages, and answer the questions in brackets: 8.1, 8.2, 9.1, 12.1, 13.1, 14.1, 14.2, 14.3, 16.1, 16.2, 18.1 - 18.8, 19.1 - 19.3, and 20.1 - 20.6. Clearly identify the question being answered and use complete sentences in your answers.
- Exercise 4: Flipping out asks you to play with the phylogenetic tree, moving the branches around and interpreting the new information it provides. Answer the questions in brackets (1.1 - 1.8) the proceed to the next exercise. Clearly identify the question being answered and use complete sentences in your answers.
- Exercise 5:Tree building Challenge. This is an optional extra credit opportunity for you. Completing the exercise will earn you 5 additional points. Answer the questions in brackets. Clearly identify the question being answered and use complete sentences in your answers.
- Exercise 6: Reconstructing Trees - As the title implies, in this activity you are to reconstruct the phylogenetic tree of columbines based on the available evidence. Answer the following questions: 8.1, 15.1, and 22.1.

You do NOT need to answer the GRADED QUESTIONS at the end of the activity. For today, submitting the answers to the questions in the workbook is sufficient evidence that you completed and understand the assignment. Questions should be answered on a separate word document and submitted via the submit button above.