Title: Unit 7 Quiz
Course: SAT 170 Introductory Biotechnology > Section: SAT 170 Introductory Biotechnology

Q: Which of the following is true regarding bacterial artificial chromosomes (BACs)?
- they are able to carry large amounts of DNA (up to 2000 kb)
- they can only be incorporated for replication and expression in bacterial host cells
- they were originally isolated from yeast
- they are single stranded
- they can only be used in genomic libraries
A: they can only be incorporated for replication and expression in bacterial host cells

Q: Which of the following correctly describes the concept known as substantial equivalence?
- a genetically modified organism has the same or very similar characteristics of another genetically modified organism
- a genetically modified organism has substantial differences when compared to an unmodified version of that organism
- a new product that has the same or very similar characteristics as an existing product or does not raise any health concerns beyond that of the existing product
- a genetically modified food or food product has the equivalent nutritional value compared to an unmodified version of that food or food product
- none of the above
A: a new product that has the same or very similar characteristics as an existing product or does not raise any health concerns beyond that of the existing product

Q: Which of the following correctly describes gene therapy?
- a technique which replaces a non-functional gene with a functional copy taken from a healthy individual
- a technique which replaces non-functional proteins with functional proteins manufactured from a healthy gene
- a technique which inserts a new gene into an organism which did not previously carry that gene
- a technique which compares the genetic composition of two individuals in order to determine the relatedness of those individuals
- none of the above
A: a technique which replaces a non-functional gene with a functional copy taken from a healthy individual

Q: Which of the following places the basic steps of protein therapy in the correct order?
- isolate a gene from a healthy individual, insert gene into individual with disease
- isolate a gene from a healthy individual, insert gene into an organism which will express that gene, collect the protein product, insert protein product into individual with disease
- isolate a gene from one organism, insert that gene into a vector, insert vector into an organism which did not previously carry that gene
- use enzymes to cut DNA at STR’s, insert digested DNA into electrophoresis gel, stain gel and observe and compare DNA fragments
- use heat to pull apart DNA strands, use enzymes and nucleotides to replicate DNA strands
A: isolate a gene from a healthy individual, insert gene into an organism which will express that gene, collect the protein product, insert protein product into individual with disease

Q: Which of the following techniques are used to detect expression of specific genes?
- Fluorescence In Situ Hybridization (FISH)
- Northern Blot
- DNA Microarray
- all of the above
- Fluorescence In Situ Hybridization (FISH) and Northern Blot only
A: all of the above

Q: Genomic DNA libraries are those which contain an organisms DNA, while cDNA libraries contain only the DNA that is expressed in a particular organism.
- True
- False
A: True

Q: Fluorescence In Situ Hybridization (FISH) can be used for both the detection of specific DNA fragments and the detection of gene expression.
- True
- False
A: True

Q: Retroviruses are the source of reverse transcriptase and can also be used as cloning vectors for DNA libraries
- True
- False
A: False

Q: ______________________________gene therapy is used to ensure that the genetic disease would not be passed on.

Q: An organism’s complete set of DNA is known as their ______________________________.

Q: ______________________________ is an enzyme that can create cDNA molecules which are synthesized using a mRNA molecule as a template.

Q: ______________________________ is a type of biotechnology which manipulates an organism’s genome.

Q: List and describe three features that make a good cloning vector.

Q: Why is mRNA used as a way of measuring gene expression?