Title: Unit 6 Review
Course: SAT 170 Introductory Biotechnology > Section: SAT 170 Introductory Biotechnology

Q: What is recombinant DNA?

Q: Compare and contrast endonucleases, exonucleases and restriction enzymes.

Q: Enzymes with nuclease activity are able to cleave the phosphodiester bond of nucleic acids.
- True
- False
A: True

Q: A _____________________ is a small, circular, double-stranded, extra-chromosomal piece of DNA commonly found in bacteria.

Q: How is electrophoresis for the separation of DNA molecules different from electrophoresis of protein molecules? (There are several differences, but one major difference was reflected in our course material).

Q: Which of the following is necessary for the polymerase chain reaction (PCR)?
- a) heat stable DNA polymerase
- b) DNA template to be copied
- c) primers
- d) nucleotides
- e) all of the above
A: e) all of the above

Q: A _____________________ is a type of extremophile (inhabit extreme environments) which thrives at high temperatures.

Q: What is the purpose of using fluctuating temperatures for the polymerase chain reaction (PCR)?

Q: _____________________ is the light that is emitted from a substance after it has absorbed light.

Q: Why must primers be included in the polymerase chain reaction (PCR)?

Q: What is the significance of using Taq polymerase as the specific DNA polymerase for the polymerase chain reaction (PCR)?

Q: When the two strands of DNA are separated for PCR, it is known as DNA melting or _____________________.

Q: Frederick Sanger is responsible for developing the first Next Generation Sequencing (NGS) technique.
- True
- False
A: False

Q: What is the role of the di-deoxyribonucleotide triphosphate molecules during the chain termination methods of DNA sequencing?

Q: Molecular cloning is a type of cloning which is used to make identical copies of a fragment of DNA.
- True
- False
A: True

Q: If the DNA segment: CCATCC, was sequenced using the Sanger Method, what would the results of that experiment looked like? Assume ddATP is red, ddGTP is green, ddTTP is black and ddCTP is blue

Q: How do “sticky ends” differ from “blunt ends” of a cut piece of DNA? Which type of cut is needed for the synthesis of recombinant DNA?

Q: How does Computer-Automated or High-Throughput Sequencing differ from the original Sanger Method of DNA sequencing?

Q: What is one application of DNA sequencing?

Q: What is one application of the human genome project?