Title: Unit 5 Review
Course: SAT 170 Introductory Biotechnology > Section: SAT 170 Introductory Biotechnology

Q: List and describe the different levels of protein structure.

Q: An organisms complete set of proteins is known as the _______________________.

Q: _______________________ are proteins that have a carbohydrate attached which function in cell recognition.

Q: Proteins are chains of _____________. The disruption of a protein’s three-dimensional shape is called ______________.
- nucleic acids; denaturation
- amino acids; denaturation
- amino acids; active site
- amino acids; tertiary structure
- nucleic acids; active site
A: amino acids; denaturation

Q: Explain the role of enzymes in our cells. Be sure to include a description of the active site and a substrate in your answer.

Q: Explain how changes to temperature and pH can lead to protein denaturation.

Q: Compare and contrast filtration and centrifugation.

Q: A _______________________ blot is performed to detect specific proteins, while a _______________________ blot is performed to identify the presence of certain protein modifications.

Q: Compare and contrast channel proteins and carrier proteins.

Q: Prokaryotes are unable to carry out many of the types of post-translational modifications (PTM’s) that occur in eukaryotes.
- True
- False
A: True

Q: Why are bacteria a good choice for upstream processing of proteins?

Q: Plants and fungi have cell walls which makes removal of proteins from them more difficult.
- True
- False
A: True

Q: A _______________________ may be used to pull apart components of a mixture using rotational force.

Q: Explain the role of the mobile phase in a chromatography experiment.

Q: What is the purpose of using a primary and a secondary antibody during the technique known as enzyme-linked immunosorbent assay (ELISA)?

Q: An eastern blot is performed to confirm the presence of specific proteins after SDS Page is complete.
- True
- False
A: False

Q: What is the role of sodium dodecyl sulfate (SDS) in SDS Page?

Q: What is the role of an electrical current during gel electrophoresis?

Q: What is upstream processing? How does this differ from downstream processing?

Q: Give two examples of post-translational modifications (PTM’s).