Title: Unit 5 Quiz
Course: SAT 170 Introductory Biotechnology > Section: SAT 170 Introductory Biotechnology

Q: Which of the following proteins is involved in cell recognition?
- enzyme
- channel protein
- carrier protein
- glycoprotein
- receptor protein
A: receptor protein

Q: Which of the following techniques relies on the use of antibodies?
- eastern blot
- enzyme-linked immunosorbent assay (ELISA)
- gel electrophoresis
- chromatography
- all of the above
A: enzyme-linked immunosorbent assay (ELISA)

Q: Which of the following techniques separates molecules such as proteins based on size?
- gel electrophoresis
- chromatography
- centrifugation
- filtration
- all of the above
A: all of the above

Q: Which of the following correctly describes the secondary structure of proteins?
- a chain of amino acids, also known as a polypeptide
- a three-dimensional globular structure composed of a single polypeptide
- a three-dimensional globular structure composed of more than one polypeptide
- a chain of amino acids that has been coiled and folded in alpha helices and beta pleated sheets
- none of the above
A: a chain of amino acids that has been coiled and folded in alpha helices and beta pleated sheets

Q: Gel electrophoresis uses a mobile and stationary phase to separate components of a mixture.
- True
- False
A: False

Q: Upstream processing refers to the isolation and purification of protein products from their host cells.
- True
- False
A: True

Q: Glycosylation is a type of post-translational modification in which a lipid is added to a specific location of a protein.
- True
- False
A: False

Q: An eastern blot is performed to confirm the presence of specific proteins after SDS Page is complete.
- True
- False
A: False

Q: The location within an enzyme to which a substrate will bind is known as the _______________________.

Q: Temperature is a measure of _______________________, which is the energy of motion.

Q: Proteins are chains of _______________________. The disruption of a protein’s three-dimensional shape is called _______________________.

Q: A _______________________ protein provides a pore or passageway, while _______________________ proteins change their shape to assist materials in getting from one side of the cell membrane to the other.

Q: What are post-translational modifications (PTM’s)? Why are prokaryotes, such as bacteria, limited in the type of PTM’s they can perform?

Q: What are the advantages and disadvantages of using a fungal host cell compared to a mammalian cell culture for upstream processing of proteins?