Title: Unit 6 Quiz
Course: SAT 170 Introductory Biotechnology > Section: SAT 170 Introductory Biotechnology

Q: What is the role of the agarose gel during the process of agraose gel electrophoresis?
- to provide the driving force that pushes/pulls the molecules to be separated
- to conduct an electrical current
- to provide a matrix that will function as a sieve for the separation of molecules
- to stain the DNA molecules
- all of the above
A: to provide a matrix that will function as a sieve for the separation of molecules

Q: Which of the following correctly describes the role of ligase in creating recombinant DNA?
- it is used to seal or “glue” together DNA fragments
- • it is used to separate two strands of a DNA molecule
- • It cuts both strands of a DNA molecule creating “blunt ends”
- it cuts a DNA molecule in a zig-zag fashion creating “sticky ends”
- none of the above
A: it is used to seal or “glue” together DNA fragments

Q: If the results of a Sanger Method DNA sequencing experiment were as follows, what would be the sequence of that DNA segment. (Assume ddATP is red, ddGTP is green, ddTTP is black and ddCTP is blue).

Fragment Length/Fragment Color
5 nucleotides/blue
4 nucleotide/red
3 nucleotides/black
2 nucleotides/green
1 nucleotide/green
- CCATG
- CATGG
- TACGG
- GGTAC
- not enough information to determine
A: GGTAC

Q: Which of the following is responsible for separating the two strands of a DNA molecule during the DNA melting, or denaturation step of the polymerase chain reaction (PCR)?
- DNA ligase
- DNA polymerase
- heat
- primers
- nucleotides
A: heat

Q: Restriction enzymes are a specific type of endonuclease.
- True
- False
A: False

Q: Recombinant DNA is a piece of DNA that is formed from combining two DNA fragments taken from different sources.
- True
- False
A: True

Q: Next Generation Sequencing (NGS) techniques are generally faster and more cost effective than older sequencing techniques.
- True
- False
A: True

Q: Agarose gel electrophoresis is used for the separation of DNA molecules, while sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS Page) is used for the separation of protein molecules.
- True
- False
A: True

Q: The specific sequence of nucleotides at which a restriction enzyme will cut is known as the _______________________________.

Q: The _______________________________ of a polymerase chain reaction (PCR) are used to initiate the process of elongation by providing DNA polymerase with something to add onto.

Q: A _______________________________ is a small, circular, double-stranded, extra-chromosomal piece of DNA commonly found in bacteria.

Q: _______________________________ is a type of cloning which is used to make identical copies of a fragment of DNA.

Q: Why is Taq polymerase so important for the polymerase chain reaction (PCR)?

Q: What is the role of the di-deoxyribonucleotide triphosphate (ddNTP) molecules during the chain termination methods of DNA sequencing?