Title: Unit 7 Review
Course: SAT 170 Introductory Biotechnology > Section: SAT 170 Introductory Biotechnology

Q: Compare and contrast genomic and cDNA libraries.

Q: List and describe three features that make a good cloning vector.

Q: Human artificial chromosomes (HACs) are the only cloning vector that can be inserted into human cells.
- True
- False
A: True

Q: Which of the following puts the cloning vectors in the appropriate order from smallest to largest?
- plasmid, yeast artificial chromosomes (YACs), bacterial artificial chromosomes (BACs), human artificial chromosomes (HACs)
- yeast artificial chromosomes (YACs), bacterial artificial chromosomes (BACs), human artificial chromosomes (HACs), plasmid
- plasmid, bacterial artificial chromosomes (BACs), yeast artificial chromosomes (YACs), human artificial chromosomes (HACs)
A: plasmid, bacterial artificial chromosomes (BACs), yeast artificial chromosomes (YACs), human artificial chromosomes (HACs)

Q: Explain the role of reverse transcriptase (RT) in producing cDNA.

Q: Describe the process known as Fluorescence In Situ Hybridization (FISH).

Q: Which of the following techniques are used to detect specific fragments of DNA?
- Fluorescence In Situ Hybridization (FISH)
- Northern Blot
- Southern Blot
- all of the above
- Fluorescence In Situ Hybridization (FISH) AND Northern Blot ONLY
A: Fluorescence In Situ Hybridization (FISH) AND Northern Blot ONLY

Q: Which of the following techniques are used to detect expression of specific genes?
- Fluorescence In Situ Hybridization (FISH)
- Northern Blot
- Southern Blot
- all of the above
- Fluorescence In Situ Hybridization (FISH) AND Northern Blot ONLY
A: Fluorescence In Situ Hybridization (FISH) AND Northern Blot ONLY

Q: Describe how a gene chip or microarray works to detect gene expression.

Q: Explain the concept of substantial equivalence.

Q: _______________________ is a DNA molecule which has been reverse transcribed from an mRNA molecule.

Q: Explain two roles of retroviruses that were discussed in this unit.

Q: What are two methods that can be used to detect genetically modified organisms (GMOs)?

Q: Compare and contrast gene and protein therapy.

Q: What is the difference between using somatic cells and gametes for gene therapy?

Q: Compare and contrast the Southern and Northern Blot techniques.

Q: Why is mRNA used as a way of measuring gene expression?

Q: Define genetic engineering.

Q: What are the basic steps for creating a genetically modified organism (GMO)?

Q: A DNA microarray is used to identify the presence of specific DNA fragments within a cell.
- True
- False
A: False